Sequence analysis and homology modeling of laccase from Pycnoporus cinnabarinus
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چکیده
منابع مشابه
Sequence analysis and homology modeling of laccase from Pycnoporus cinnabarinus
Industrial effluents of textile, paper, and leather industries contain various toxic dyes as one of the waste material. It imparts major impact on human health as well as environment. The white rot fungus Pycnoporus cinnabarinus Laccase is generally used to degrade these toxic dyes. In order to decipher the mechanism of process by which Laccase degrade dyes, it is essential to know its 3D struc...
متن کاملHighly efficient production of laccase by the basidiomycete Pycnoporus cinnabarinus.
An efficient transformation and expression system was developed for the industrially relevant basidiomycete Pycnoporus cinnabarinus. This was used to transform a laccase-deficient monokaryotic strain with the homologous lac1 laccase gene placed under the regulation of its own promoter or that of the SC3 hydrophobin gene or the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of Schizophyllum...
متن کاملSelection of Pycnoporus cinnabarinus strains for laccase production.
A comparison of Pycnoporus cinnabarinus strains for laccase production was carried out. A dikaryotic strain, I-937 strain, producing a high level of laccase (9500 U l(-1)) was selected. The study of the life cycle in vitro of this dikaryotic strain led to isolation of monokaryons. Forty-eight monokaryotic strains were isolated and screened for laccase production. One of these strains, ss3, prod...
متن کاملMolecular analysis of a laccase gene from the white rot fungus Pycnoporus cinnabarinus.
It was recently shown that the white rot basidiomycete Pycnoporus cinnabarinus secretes an unusual set of phenoloxidases when it is grown under conditions that stimulate ligninolysis (C. Eggert, U. Temp, and K.-E. L. Eriksson, Appl. Environ. Microbiol. 62:1151-1158, 1996). In this report we describe the results of a cloning and structural analysis of the laccase-encoding gene (lcc3-1) expressed...
متن کاملThe ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase.
The white rot fungus Pycnoporus cinnabarinus was characterized with respect to its set of extracellular phenoloxidases. Laccase was produced as the predominant extracellular phenoloxidase in conjunction with low amounts of an unusual peroxidase. Neither lignin peroxidase nor manganese peroxidase was detected. Laccase was produced constitutively during primary metabolism. Addition of the most ef...
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ژورنال
عنوان ژورنال: Bioinformation
سال: 2010
ISSN: 0973-8894,0973-2063
DOI: 10.6026/97320630005150